Abstract

IDENTIFICATION FINGERPRINT TO ASPERGILLUS NIGER ISOLATES USING RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) ANALYSIS

Qadisiyah H. Hashoos * and Alaa M.Y. Al-Araji

Abstract

The present study was aimed to identify of Aspergillus niger isolates isolated from different sources (soil, peanut, milk and factory waste water) by traditional methods and find the DNA fingerprint of isolates by using RAPD technique. Identification of twenty four Aspergillus niger isolate by using traditional methods (macroscopic and microscopically). Genomic DNA of each isolates was extracted at a final concentration of 115-959μg / 2-3g of wet mycelium, and at a purity of 1.7-1.9. Each DNA sample was amplified with each of 12 primers and the products were resolved electrophoretically on 1.2% agarose gel, stained with ethidium bromide and photographed under UV. Five Primer failed to support amplification, while another seven primers succeeded in that. RAPD analysis fingerprinted nineteen of the twenty four isolates through marker bands with one or more of the 7 primers.

Keywords: Aspergillus niger, DNA, RAPD, Fingerprinting.