Abstract

IN VIVO PRELIMINARY BIOCHEMICAL SCREENING OF ETHNO ANTI-DIABETIC PLANTS DEPLOYED IN SOUTH-EAST RAJASTHAN (INDIA)

Asha Arora* and Vinita Paliwal

Abstract

medicinal survey with reference to hypoglycemic activity was carried out in various ethnic pouches of south east Rajasthan and on the basis of % fidelity level, 25 plants were selected and subjected to preliminary in vivo biochemical screening through oral glucose tolerance test (OGTT). Aqueous extract of respective plant part was orally ingested prior to intragastically glucose loaded wistar rats and blood glucose level was registered after 0, 30, 90 and 120 mins through glucose oxidase and peroxidase (GOD / POD) method. Glibenclamide (GBS) was used as a reference drug to mark the efficacy of plant. Percent variation of glucose level over initial time (% Gvt) and percent variation of glucose level with respect to reference drug (%Gld) were studied for all respective time intervals in all groups to mark the anti-hyperglycemic efficacy of the plant.Glucose levels with respect to initial time declined in groups treated with Aerva lanata, Aloe barbadensis, Andrographis paniculata, Cassia sophera, Catharanthus roseus, Cayratia trifolia, Citrullus colocynthis, Cyamopsis tetragonoloba, Dalbergia sissoo, Gymnema sylvestre and Phyllanthus emblica. Groups injested with phytoextract of Aerva lanata, Andrographis paniculata, Boerhaavia diffusa, Catharanthus roseus, Cayratia trifolia, Costus speciosus, Cyamopsis tetragonoloba, Gymnema sylvestre and Phyllanthus emblica were akin to GBS. On the array of both the variables i.e. declivity over time and with reference to GBS Aerva lanata, Andrographis paniculata, Catharanthus roseus, Citrullus colocynthis , Cyamopsis tetragonoloba and Gymnema sylvestre were found to be efficacious but the glucose level of Aerva lanata at source point i.e. 0 min was comparatively very high and statistically not significant.

Keywords: South east Rajasthan, Diabetes, OGTT, Andrographis paniculata, Catharanthus roseus, Citrullus colocynthis, Cyamopsis tetragonoloba, Gymnema sylvestre.