Abstract

ANTIBACTIREAL ACTIVITY OF CUSCUTA REFLEXA (CONOVOLVULACEAE) AGAINST HUMAN PATHOGENIC MICROBIAL STRAINS

Dhanendra Kumar Rai*, Vibhu Sharma, Anju Pal and Krishan Pal

Abstract

Cuscuta reflexa is a parasitic plant. It is used as a purgative, constipation, alternative in different disorder. Antimicrobial agent are in the treatment of infections because of their selective toxicity that is they have ability to kill an microorganism without harming the cells of the host. Antibacterial activity of leaves of Cuscuta reflexa were studied using ethanolic extract against Gram positive bacteria like Streptococcus mutants, Staphylococcus aureus and Gram negative bacteria like Escherichia coli, Klebsiella pneumonia, Solmonella typhi, Proteus mirabilis, Staphylococcus aureus. Using Standard antibiotic tetracycline. Antibacterial activity was tested using a modification of the disc diffusion method. The etahanolic extract were poured into the well of sterile nutrient agar medium using agar cup plate technique. The result were analysed by using zone of inhibitions and it was observed that Gram negative and Gram positive strains showed more antibacterial activity as compared to the Gram positive bacteria.

Keywords: Cuscuta reflexa, Antimicrobial activity, Ethanolic extract, Microorganism.