Abstract

RESTRICTION DIGESTION ANALYSIS OF GENOMIC DNA OF THE CESTODE PARASITE, Raillietina Tetragona INFECTING THE INTESTINE OF THE COUNTRY FOWL (Gallus Domesticus)

J. Manju Bashini, P. Senthilkumaar* and T. Radha

Abstract

Economic losses due to helminth parasites in country fowl throughout the world are considerable. Though less emphasis has been placed on utilising DNA elements to study cestodes of veterinary importance, correct diagnosis is, nevertheless, critical to proper treatment and control. These are able to induce structural, biochemical and immunological changes in the host like inappetence, diarrhoea, dull hair coat and ostertagiosis. At present, control is almost exclusively based on anthelmintics. Hence, Genomic DNA analysis plays an increasingly important role in characterising and classifying cestode parasites. The present study revealed restriction digestion analysis of genomic DNA of Raillietina tetragona reference isolates collected from country chicken “Gallus domesticus” from Zambazaar market, Triplicane, Chennai (Tamil Nadu) using 4 restriction enzymes revealed some differences in the restriction profile of DNA in the chick isolates. Bgl I and Pvu II restriction digestion showed bands above 23Kbs of Lambda Hind III marker and streak formation below 2Kbs. Dra I showed a single band above 23 Kbs of lambda Hind III marker. No restriction site and bands could be fixed unambiguously with restriction enzyme Hinf I.

Keywords: Country chicken, Raillietina tetragona, Genomic DNA analysis, Restriction enzymes.