Abstract

ANALYTICAL METHOD DEVELOPMENT FOR DETERMINATION OF EUGENOL CONCENTRATIONS IN SELECTED SPECIES OF OCIMUM

*Amit Joshi, A.K. Pathak, Mukul Tailang

Abstract

Eugenol is used as a flavor in the food industry, has a variety of biological activity, and can serve as a biomarker. Because eugenol is present in the leaves of Ocimum, which are used as an herbal medicine, a sensitive and reliable quantitative Ultra-violet spectroscopy and highperformance liquid chromatographic method has been established for quantification of the compound in the leaves of the plant. A methanol extract of the powder of dried leaves of Ocimum, was spotted on the Merck aluminium plate precoated silica gel F254 with 0.2 mm thickness. Meoh:Chloroform (95:5) and MeOH: H2O: ACN (50:25:75) used as mobile phase to isolate the eugenol and to prepare the sample for UV and HPLC analysis respectively. The UV and HPLC method proposed for the quantitative monitoring of eugenol in Ocimum leaf powder is rapid, simple, and precise. Hence from that UV and HPLC analysis it was concluded that the Ocimum sanctum linn contains higher concentration of Eugenol.

Keywords: Ocimum; Ultra-violet spectroscopy; high-performance liquid chromatography; Merck aluminium plate precoated silica gel.