Abstract

EFFECT OF GEOGRAPHICAL DISTRIBUTIONS ON THE PHYTOCHEMICAL PROFILE OF METHANOLIC EXTRACT OF DRIED LEAVES OF ROSMARINUS OFFICINALIS L.

N. Chandralega, D. Subha and N. Geetha

Abstract

Rosmarinus officinalis L. (Rosemary) is a medicinal herb belongs to Lamiaceae family have shown a variety of pharmacological activities as cancer chemoprevention and therapy using in vitro and in vivo models. The study was conducted to the herb that was chosen from different locations (named as RO1, RO2, RO3, RO4 and RO5) with respect to qualitative and quantitative phytochemical analysis. Preliminary phytochemical screening reveals that the presence of phytoconstituents including alkaloids, phenols, cardiac glycosides, saponins, flavonoids, tannins, terpenoids, steroids, carbohydrates, proteins, coumarins and quinines, while anthraquinones was absent in all the samples collected from different locations of kodaikanal hills. RO3 sample was found to contain high level of phenolic content (62.39 mg/g) followed by RO2 (55.53 mg/g) and RO1samples (53.51 mg/g), while RO4 (44.36 mg/g) and RO5 samples (40.75 mg/g) contain low level of phenolics. The total flavonoid content was found to be maximum (35.18 mg/g) in RO3 followed by RO2 (26.17mg/g) and RO1 (23.28 mg/g) samples and minimum in RO4 (18.94 mg/g) and RO5 (17.75 mg/g) samples. The total tannin content was found to be maximum (29.70 mg/g) in RO3 followed by RO2 (22.28 mg/g) and RO1 (21.48 mg/g) and minimum in RO4 (18.16 mg/g) and RO5 (15.86 mg/g). The total carbohydrate content was maximum (31.85 mg/g) in RO3 followed by RO2 (24.88 mg/g), RO1 (23.75 mg/g), RO4 (19.92 mg/g) and RO5 (17.64 mg/g). The protein content was maximum (25.20 mg/g) in RO3 followed by RO2 (18.16 mg/g), RO1 (16.00 mg/g), RO4 (13.18 mg/g) and RO5 (11.59 mg/g).

Keywords: Rosmarinus officinalis L., Lamiaceae, Phytoconstituents, Medicinal herb.