Abstract

ANTI TUMOR ACTIVITIES OF SECRETION EXTRACT OF BELLAMYA BENGALENSIS IN HUMAN HEPATOCELLULAR CARCINOMA CELL LINES IS MEDIATED BY CASPASE-DEPENDENT APOPTOSIS AND CELL CYCLE ARREST

Abhishek Ranjan Besra, Krishnendu Pal, Santanu Basu, Shila Elizabeth Besra

Abstract

Anti leukemic activity of secretion extract of Bellamya bengalensis f. Annandalei is already reported. We evaluated the cytotoxic and apoptosis inducing effect of secretion extract of Bellamya bengalensis (SEBB) on hepatocellular carcinoma cell lines and NO assay on mouse murine macrophage cell line. It was found that SEBB significantly decreased the metabolically viable cells and that SEBB caused apoptosis, as confirmed by morphologically and by Flowcytometric analysis shows appreciable number of cells in early & late apoptotic stages and cells are arrested in the sub-G1 & G1 phases of cell cycle. The SEBB induces nitric oxide in RAW264.7 cells but when used in combination with recombinant interferon-γ (rIFN-γ), it shows a marked enhancement in NO production in murine macrophages RAW264.7 cells. Elevated NO production was significantly inhibited by pre-treatment with PDTC and NGMMA, thus SEBB indicates a role of immunomodulation.MTT assay in RAW264.7 cells revealed that insignificant reduction and apoptosis is mediated through activation of caspase 3.

Keywords: Hepatocellular Carcinoma, Bellamya bengalensis, Secretion, Apoptosis, Nitric Oxide.