Abstract

ESTERATIC ACTIVITY DEMONSTRATION ON HUMAN GASTROESOPHAGEAL REGION

Mohammad Oda Selman*

Abstract

Background: Histochemical demonstration of the smooth muscle fibers in the gastro-esophageal region, esteratic activity was used to demonstrate the muscle fibers activity and differentiation. Objectives: To identify the site of activity of muscle fiber in the gastro esophageal region. Material and Method: Full thickness specimens was taken from GEJ of three different adult male human cadavers from the central forensic institute to demonstrate the esteratic activity by asodye coupling method then the optical density was measured. Result: Different areas were chosen to show that the activity of the muscle fiber located in Z-line region and the region above and below by four cm. Staining of muscle sections is simultaneous azo-dye coupling method in which-naphthyl acetate act as substrate for esterase group of enzyme. This staining with ANAE method showed clear increased muscle activity in the region of lower esophagus (above Z-line) more than the region below Z-line and the least in the Region of Z-line. Full automatic computerized software was used to specrtophotomorphometric evaluation (global lab image/2). Conclusion: Depending on esteratic activity the muscular layer of the gastro-esophageal junction shows that the sphinecteric action in the lower esophagus related with the high esterase activity of smooth muscle fibers in this region which is apparently clear in histochemical esteratic activity when the different sections dealt with non specific ANAE and the transitional Zregion area just rooming area for food stuff. The Z-area smooth muscle had the least activity after studying the morphometry of the biopsies taken from these areas.

Keywords: Z-line, optical density, ANAE, polyvinyl pyrrolidone (PVP).