Abstract

ANAVEL APPROACH FOR SPECTROPHOTOMETRIC DETERMINATION OF ROXYTHROMYCIN

Mallapu. E. Rani

Professor, Head of the Dept of Chemistry, Rayalaseema University, Kurnool, A.P. India.

Abstract

This paper describes the validation of a developed a new spectrophotometric method for the determination of Roxythromycin in pharmaceutical for mulations. In this method is based on the reaction of roxythromycin with 2,3 – dichloro – 5,6 dicyano – 1,4 – benzoquinone (DDQ) in acetonitrile mixture to form dark red colour charge transfer complex solution. This dark red colour solution was stable for more than 24hrs and was used to detcrmine the roxy thromycin spectrophotometrically. The absorbance of the dark red colour solution was measured at the wave length range of 400 to 600nm, against the reagent blank. The calibration graph was obtained by plotting absorbance values against the concentrations of roxythromycin solution. The calibration curve was found to be linear over a concentration range of 50-250  g / ml roxythromycin. The linearity of the curve obtained indicates that it obeys Beer’s law. Results of analysis were validated statisticaly and by recovery studies. The procedures deseribed were successfully applied to the determination of roxythromycin in tablets.

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