Abstract

ANTIMICROBIAL AND ANTI ADHESIVE PROPERTIES OF PRODIGIOSIN EXTRACTED FROM SERRATIA MARCESCENS AGAINST BACTERIA ISOLATED FROM PATIENTS WITH LEUKEMIA.

Dr. Taif H. Hussan* and Sawsan H. Authaman

Abstract

In this study, Prodigiosin extracted from Serratia marcescens. The optimum condition of pigment production including growing at different temperature for different incubation periods of times were studied. Aerobic condition for 72 hours at 30 C° was fitting to Prodigiosin production. Additionally, Prodigiosin was extracted by Chloroform and methanol and purified by acid precipitation and using Thin layer Chromatography method. Prodigiosin activity were studied, antimicrobial, anti adhesive and antibiofilm activities were evaluated against some cancer infection causative bacteria including Gram positive and Gram negative types , isolated from blood samples of Iraqi patients suffering from leukemia. Prodigiosin showed inhibitory effect against leukemia causative bacteria at concentration (0.2) mg/ml against all these bacteria and showed inhibitory effect on adherence and biofilm formation of these bacteria, E. coli more sensitive to Prodigiosin, when gave the highest inhibitory percentage (46.5) % followed by Pantoea sp. (37.3) %, while A. baumanii was more resist (inhibitory percentage 2.7%).

Keywords: Prodigiosin, Serratia, Antimicrobial, Anti adhesive, Leukemia.