Abstract

ANTIOXIDANT EFFECT OF RADISH (RAPHANUS SATIVUS L.) AND LEEK (ALLIUM PORRUM L.) JUICES AGAINST HEPATOTOXICITY AND NEPHROTOXICITY INDUCED BY DIMETHOATE IN MALE ALBINO MICE

Gehan Salah Eldin Moram*, Tahany El-sayed Kholief, Aziza Tarik Fathy Ahmed

Abstract

Objectives: This study aimed to investigate the ameliorative effect of radish and leek (leaves and roots) juices against the damage induced by dimethoate (DM) on liver and kidney functions and oxidative stress in mice. Methods: DM was administered orally at a dose of 10mg/kg b.wt /day. Radish and leek (leaves and roots) juices were administered orally at a dose of 8ml/kg b.wt /day. Mice were divided into 10 groups: G1: healthy control, G2: radish leaves juice (healthy RLJ), G3: radish roots juice (healthy RRJ), G4: leek leaves juice (healthy LLJ), G5: leek roots juice (healthy LRJ), G6: dimethoate group (DM), G7: RLJ plus DM, G8: RRJ plus DM, G9: LLJ plus DM, G10: LRJ plus DM. Results: The results indicated that DM depleted the oxidative stress markers including reduced glutathione content (GSH), superoxide dismutase activity (SOD), and total thiol content (T.SH) and also significantly elevated malondialdehyde level (MDA), nitric oxide concentration (NO) and advanced oxidation protein products content (AOPP) in liver and kidney tissues. However, radish and leek (leaves and roots) juices significantly elevated the depleted oxidative stress markers including GSH, SOD and T.SH and also significantly reduced the marked elevation of MDA, NO and AOPP. Moreover, radish and leek juices significantly improved liver, kidney functions and hematological parameters in mice administered dimethoate. These results were confirmed by histopathological examination of liver and kidney. In conclusion, Radish and leek juices administration attenuated the severity of oxidative damage accompanying dimethoate toxicity with hepatoprotective and nephroprotective properties.

Keywords: Radish, leek, dimethoate, antioxidant, hepatoprotective, nephroprotective.