Abstract

PRELIMINARY PHYTOCHEMICAL SCREENING AND HPLC ANALYSIS OF BARK AND LEAF EXTRACT OF DOLICHANDRONE ATROVIRENS

Saminathan Kayarohanam* and S. Kavimani

Abstract

The present paper deals with the phytochemical screening of bark and leaf extract of Dolichandrone atrovirens an important medicinal plant used in the Indian traditional system of medicine. This study involves the preliminary screening of carbohydrates, glycosides, protein, aminoacids, tannins, phenol, terpenoids, flavonoids, saponins, fixed oil and fats. Dolichandrone atrovirens leaf and bark extracts are further analyzed by HPLC using Shimadzu Class-VP V6.14 SP2 system.The preliminary screening test results in the detection of bioactive principles and HPLC analysis conform the phytochemical active ingredients like gallic acid, rutin, quercetin, and ferulic acids are present in the leaf and bark extracts of Dolichandrone atrovirens.This analytical technique used to find the pharmacologically active constituents present in the aqueous methanolic bark and leaf extract of Dolichandrone atrovirens.

Keywords: Aqueous methanolic, HPLC, bark and leaf extract, Dolichandrone atrovirens.