Abstract

DEVELOPMENT AND VALIDATION OF HPTLC METHOD FOR GLYCYRRHIZIN AND ITS APPLICATION FOR HERBAL ANTIACNE GEL

Pratibha Nand*, Sushma Drabu, Rajinder K Gupta

Abstract

Objective: Number of analytical techniques are available for estimation of glycyrrhizin in extracts and herbal formulations. To our knowledge, there is no specific HPTLC (high-performance thin layer chromatography method) for quantification of glycyrrhizin in herbal gel compositions. In the present study HPTLC method was developed, validated and used for fingerprinting of glycyrrhizin in anti-acne formulation. Methods: Physicochemical, phytochemical and antibacterial potential of Glycyrrhiza glabra was assessed. A simple HPTLC method was validated. Method comprised application of 5 μl sample on 60GF254 plate and was placed in twin trough glass chamber (10 cm×10 cm) having solvent system of Pet ether: Benzene: Ethyl Acetate: Glacial Acetic Acid (4:8:2.8:0.2, v/v/v/v) and quantification was done using TLC scanner and in UV chamber at wavelength of 254 nm. Results: This system was found to give compact spots for glycyrrhizin (Rf value: 0.38 ± 0.02). A strong linear relationship between the peak area and concentration of glycyrrhizin was observed with correlation coefficient (r2 = 0.99021 ± 7.470210). The method was validated for precision, accuracy, recovery and robustness. Amount of glycyrrhizin present in herbal extract and gel formulation was quantified as (7.934 %) and (1.070 %) respectively at 254 nm. Conclusion: It was a simple, sensitive, precise and reproducible method and can be used for quality assurance of the herbal composition containing glycyrrhizin.

Keywords: Glycyrrhiza glabra, glycyrrhizin, HPTLC, validation, Gel.