Abstract

EVALUATION OF IN-VIVO ANTIOXIDANT EFFECT OF ETHANOLIC LEAF EXTRACTS OF GREWIA UMBELLIFERA STREPTOZOTOCIN (STZ)-INDUCED DIABETIC RATS

S. Gunasekaran*, V. Srinivasan , R. Panneerselvam and S. Palani

Abstract

The aim of the present study is to investigate the in vivo antioxidant activities Ethanolic Leaf Extract of Grewia Umbellifera (ELEGU) in streptozotocin-induced diabetic rats. Animals were treated with ELEGU for 28 days and then diabetic and oxidative stress was induced with a single dose of streptozotocin 50mg/kg. Treated with 250 mg/kg body wt and 500mg/kg body wt of ELEGU on hepatic and renal thiobarbituric acid reactive substances, hydroperoxides, GSH, SOD, CAT, GPx, GST, and NO enzymes levels was determined. The present study revealed that Grewia Umbellifera has significant in-vivo antioxidant activity and can be used to protect tissue from oxidative stress. The result shows a significant decrease in hepatic and renal Thiobarbituric acid substances and Hydroperoxides. The treatment also resulted in a significant increase in liver and kidney GSH, SOD, CAT, GPx, GST and NO when compared with diabetic control groups. Ethanolic Leaf extract of Grewia Umbellifera in the dose of 250mg/kg and 500mg/kg as well as glibenclamide, have improved the GSH, SOD, CAT, GPx, GST levels significantly, which were comparable with diabetic induced rats. Based on this study we conclude that the Grewia Umbellifera possesses in vivo antioxidant- activity and can be employed in protecting tissue from oxidative stress.

Keywords: Grewia Umbellifera, Thiobarbituric acid, Hydroperoxides.