Abstract

IN-VITRO ANTIMICROBIAL EFFECT OF ADHATODA VASICA, ANNONA SQUAMOSA, ALOE VERA, BUTEA MONOSPERMA AND PROSOPIS JULIFLORA

Patel Harshad B.*, Patel Urvesh D., Modi Chirag M., Javia Bhavesh B., Bhatt Punit R., Pandya Kajal B.

Abstract

In-vitro antibacterial activity of aqueous, alcoholic, and chloroform extracts of leaves of Adhatoda vasica, Annona squamosa, Butea monosperma, Prosopis juliflora and Aloe vera leaf pulp against various animal bacterial isolates was investigated by disc diffusion assay. Different extracts were also screened qualitatively for the presence of active phytochemicals and total extractability was also calculated. Amongst different plant extracts P. juliflora methanolic extract has shown good antibacterial activity against B. cereus, E. coli, P. vulgaris, S. aureus and S. agalactiae except S. typhimurium. Methanolic extract of the plant has shown highest mean zone of inhibition (mm) 23.13 ± 0.34 against S. agalactiae. This extract was also significantly effective against B. cereus. Methanolic extracts of A. squamosa and B. monosperma were effective against E. coli with mean zone of inhibition (mm) 22.73 ± 1.17 and 19.70 ± 0.87 respectively. A. vasica water extract has shown little activity against S. agalactiae. Phytochemical analysis has revealed variation in presence of alkaloids, triterpenes, tannins, steroids, flavonoids, glycosides, saponins, and carbohydrates in different extracts of plants. Methanolic extract of Prosopis juliflora has shown presence of alkaloid that might have resulted in antimicrobial effect of plant which should be further explored for its in-vivo efficacy in animal infections.

Keywords: Antimicrobial activity, Phytochemical analysis, Adhatoda vasica, Annona squamosa, Butea monosperma, Prosopis juliflora, Aloe vera.