Abstract

IN VITRO MICROPROPAGATION OF COLDENIA PROCUMBENS L. FROM NODAL EXPLANTS

G. Jahirhussain*, K. Kala, S. Alagumanian, V. Muniappan, V. Tamilselvan, P. Rajkumar

Abstract

An efficient protocol for in vitro micropropagation of Coldenia procumbens L. has been developed. The nodal explants were cultured on MS basal medium supplemented with different concentrations of BAP and KIN. The two cytokinins tested, BAP was found to develop in shoot multiplication and higher number of shoots from the nodal explants when compared to KIN. higher number of shoots was produced from all the concentrations of both BAP and KIN. The highest frequency of 100% shoot induction was observed on MS basal medium supplemented with 10 μM BAP and 8 μM KIN. The number of shoots observed on the MS basal medium supplemented with BAP obtained 15 as well as 10.6 in KIN. The data's are recorded after 30days of inoculation.

Keywords: Coldenia procumbens L., cytokinins, nodal explants.