Abstract

IN VITRO ANTIOXIDANT AND PHYTOCHEMICAL SCREENING OF METHANOLIC EXTRACT OF AGARICUS BISPOROUS (BUTTON MUSHROOM)

R. Rajasekar* and K. Jeyaprakash

Abstract

Objective: The present study was carried out to evaluate the qualitative and In vitro antioxidant activities of methanolic extract of Agaricus bisporous (button mushroom) Materials And Methods: Agaricus bisporous was extracted with three solvents like methanol,ethanol and chloroform. Three extracts of Agaricus bisporous were tested for different phytoconstituents and the In vitro antioxidant activity of the methanolic extract was studied by using 1,1-diphenyl-2-picryl hydrazyl (DPPH) radical scavenging activity, Reducing power activity, hydrogen peroxide scavenging activity, Superoxide scavenging activity and nitric oxide scavenging activity. Results: The yield of phytochemicals is in the order of methanol extract > ethanol extract >chloroform extract were obtained. Finally, methanolic extract was selected for further investigation. Highest free radical scavenging activity of DPPH assay, reducing power scavenging activity, superoxide radical scavenging activity, hydrogen peroxide scavenging activity and nitric oxide radical scavenging activity, was found to be 84.41±5.90 μg/ml, 0.78±0.05μg/ml, 82.30±5.76μg/ml, 72.50±5.07μg/ml, 84.23±5.89μg/ml, at highest concentration of methanol extract of 80 μg/ml respectively in this study. Conclusion: The present study concluded the in vitro antioxidant activity of Agaricus bisporous which might be due to the presence of the phenolic and flavonoid compounds.

Keywords: Agaricus bisporous, Mushroom, Phytochemical, In vitro antioxidant, scavenging activity.