Abstract

DEVELOPMENT AND VALIDATION OF UV SPECTROPHOTOMETRIC AND RP-HPLC METHODS FOR THE DETERMINATION OF BISOPROLOL FUMARATE IN PURE AND PHARMACEUTICAL DOSAGE FORM

*Manimala.M¹, Ravindra Reddy.K¹, Hepcykala Rani.D¹

P. Ramireddy Memorial College of Pharmacy-Kadapa, -516 003, Andhra Pradesh, India.

Abstract

The aim of the study was to develop UV-spectrophotometric and RPHPLC methods for the analysis of Bisoprolol in marketed tablets and pure form.The methods were validated in terms of linearity, accuracy (%Recovery), precision (inter day, intraday and reproducibility) and robustness. Both the methods were linear (R2 =0.999 for UV method and 0.999 for RP-HPLC method) and accurate.The methods was also found precise (% RSD< 2%) and robust.All validation parameters were within the acceptable range as perInternational Conference on Harmonization (ICH). Parameters of validation prove the precision of the method and its applicability for the determination of Bisoprolol in pharmaceutical tablet formulations.

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