Abstract

THE ANTI-ULCEROGENIC ACTIVITY OF THE CRUDE METHANOLIC EXTRACT OF BERGIA SUFFRUTICOSA

Amna Elhassan Hamad Mohammad, Osama Yosuf Mohammed, Sania Abdallah Shaddad, Ahmed Mohamed Ibrahim Hamad.

Abstract

This study was carried out to evaluate the anti-ulcerogenic activity of the methanolic extract of Bergia suffruticosa to validate its traditional uses in treatment of stomach disturbances. The methanolic extract of Bergia suffruticosa was screened for its anti-ulcerogenic activity against induced-ulcer in four different models. The phytochemical studies of the plant showed that flavonoids,sterols, triterpenes, tannins, saponins and cuomarins are the main conistituents of the plant. The results showed that the methanolic extract of Bergia suffruticosa at a dose of 300 mg/kg was markedly decreased the incidence of ulcer that induced by aspirin in rat stomach and reduced ulcer index from 19.1±1.6 that induced by 150mg/kg aspirin to 10.8±07. Also inhibited H+ion concentration that had been stimulated by administration of histamine (2μg/kg) in rat stomach when the plant was given simultaneously at a dose 400ug/kg,with histamine and elevate the pH to 4.34±1.03 compared with the pH 3.14±1.20 that induced by administration of 2ug/ml histamine alone. Also B.s extract was antagonized histamine effect in contracting rat uterus and blocked the stimulant effect of histamine on guinea pig atrium.Also the extract antagonized ulcer induced by HCl/ethanol in mice by40.09%ulcer inhibition, compared with 50.54% that of sucralfate. The extract reduced water immersion stress induced ulcer in rat by 47.59%ulcer inhibition whereas that of omeprazole was 100%.The results obtained from this study confirmed the anti-ulcerogenic activity of the methanolic extract of Bergia suffruticosa.

Keywords: Anti-ulcer; A Bergia suffruticosa; spirin-induced ulcer; H+ ion concentration.