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Abstract

ADOPTION OF CYTOCHROME B AS A MEANS TO IDENTIFY THE SOURCE OF DNA USING POLYMERASE CHAIN REACTION

Basma M. Arif ? and Ikbal K. Abbas

Abstract

The present study was aimed to determine the type of biological material of unknown origin through the cytochrome b gene besides PCR, and this is important for samples for forensic medicine lawsuits involved in the investigation Criminal, as the forensic task to resolve the issues in routine casework, sometimes it is necessary to identify the exact species of the biological material. Samples, which included several isolates of Pseudomonas aeruginosa and blood of different animal sources included (rabbits, chickens, sheep), human blood and corpses tissue. It was detected amplification of the gene coding for the types of product covered by the study, where P.aeruginosa gave the package, which appeared the size of 460 base pairs. As was detected amplification of the gene coding for the following kind of product (Sheep, chickens, rabbits), where the given package size 331, 227, 740 base pairs respectively. The product amplification of the gene coding for the type of human gave specialist pack size of 580 base pairs for each of the blood and corpses tissue samples.

Keywords: polymerase chain reaction, cytochrome b, Pseudomonas aeruginosa, corpses tissue.


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