Abstract

IN VITRO RAPID MULTIPLICATION OF SOLANUM TRILOBATUM L. FROM SHOOT TIP EXPLANT

S. Subbaiya*, S. Alagumanian, G. Jahirhussain, T. Nagarajan

Abstract

An efficient protocol was developed for in vitro regeneration of Solanum trilobatum (L.) is a perennial deciduous armed shrub belonging to the family solanaceae. Shoot tip explants of Solanum trilobatum were cultured on MS basal medium supplemented with different concentrations of BAP and KIN ranging from 0.5 - 2.5 μM/L for multiple shoot induction. The two cytokinins tested, BAP was found to develop in shoot multiplication and higher number of shoots from the shoot tip explants when compared to KIN. Higher number of shoots was produced from all the concentrations of both BAP and KIN. The highest frequency (100%) of shoot induction and maximum number of shoot (8.4±1.51) was observed on 2.0 μM BAP with shoot length of 4.94±0.20 c.m. in KIN 1.5 μM/L to produce maximum number of shoots (6.4±1.81) in the shoot length of 4.66±0.37 and the shoot induction frequency is 100%. The isolated shoots were transferred to MS basal medium supplemented with different concentrations of IBA and NAA ranging from 0.5 - 2.5 μM/l for root induction. The rooted plantlets were successfully transferred in soil through hardening and established in the field.

Keywords: Micropropagation, Solanum trilobatum, BAP, IBA.