Abstract

IN-VITRO AND IN-VIVO ANTI-INFLAMMATORY POTENTIAL OF TABERNAEMONTANA DIVARICATA LEAVES

Sumithra M*¹, Chitra.V¹, Anbu J², Arijit Chakraborty²

Abstract

Tabernaemontana divaricata is an evergreen shrub traditionally used as Anti-inflammatory, analgesics and sedative effect. In the current study, an attempt has been made to prove the anti-inflammatory potential of Tabernaemontana divaricata leaves in in-vitro Human red blood cell membrane stabilization method, and in-vivo carrageenin induced anti-inflammatory model. The doses were fixed after performing acute toxicity study according to the OECD guideline 423. Diclofenac sodium was administered as standard drug for both in-vitro and in-vivo study. In case of in-vitro study blood samples were collected and centrifuged. Then supernatant fluid was collected and mixed with standard and test drugs. Then the reaction mixture was centrifuged and collected supernatant was subjected for UV analysis at 560 nm and percentage inhibition of Haemolysis was calculated. In in-vivo method carrageenin induced paw edema model was evaluated using rats. 200mg/kg and 400mg/kg dose were orally given to the test groups once daily for a month. Edema was produced injection of carrageenin by sub-plantar route in the right hind paw of each rat one hour after the administration of corresponding drugs. The paw volume was measured by using the plethysmometer. Mean increase in the volume of oedema was measured and the percentage inhibition was calculated. After performing those two models, we can conclude that the ethanolic extract of Tabernaemontana divaricata leaves was reduced the inflammation as compared with the standard drug diclofenac sodium.

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