Abstract

RESPONSE OF CADAVERINE ON THE CULTURED TISSUES OF BRASSICA JUNCEA (RH-30)

Pushpa C. Tomar*, Abhilasha Shourie, Anil K. Haritash, Shyam N. Mishra

Abstract

Cadaverine (Cad), out of two diamine of growth modulating polyamines which is characteristics synthesize entirely from different pathway with respect to the group, anticipated in growth of the plant, but antistress role is obscure. The Cad effect on germination and plant growth under various stress regimes (from explants hypocotyls of Indian mustard Brassica juncea RH-30) The tissue culture media was MS induced with metal Cd and Pb (1mM) and NaCl salinity was 100mM. The explants under Cad media showed better growth over control and stressed one. Further, subculturing of callus in the presence of Cad maintained high callus growth over control and even with NH4NO3 supplementation. However, Cad did not check the inhibitory effect of salinity and Pb stress on sub-cultured callus growth. On the other side supplementation of Cad did not show any stimulatory effect on nitrate reductase activity except that a little increases in activity with salt and Pb presence. Cad ameliorates the growth of the callus under stress conditions and these stress induced proteins in the presence of Cad may help plant to grow under stress condition.

Keywords: Brassica juncea, Cadaverine, Metal, Salinity, Stress.