Abstract

ASSESSMENT OF ANTIBIOTIC SUSCECPTIBILITY PATTERN OF GRAM NEGATIVE PATHOGENS ISOLATED FROM INTENSIVE CARE UNIT PATIENT OF “COPD” IN SOUNTH ZONE OF TAMILNADU, INDIA.

S. Vasuki Srinivasan* and S. Ahemed John

Abstract

Extended spectrum of Beta-lactamase (ESBL) and emergence of antibacterial resistance among nosocomial pathogens in the intensive care unit of hospitals created serious health care concern. The resistance developed by G-ve bacteria against Beta-lactam antibiotics. The aim of the study to evaluate the prevalence of multidrug resistance organism and incidence of ESBL producers in G-ve bacteria isolated from endotreacheal secretion of COPD patient of private hospital, Tamilnadu. 289 tracheal secretion samples collected and screened for the presence of bacterial pathogens. The pathogens were identified using selective media technique and confirmed with Hi-chrome agar and automatic technique biomerius. The ESBL producer’s screened by double disc synergy test. Among 289 samples, 232 showed culture positive and 57 samples showed culture negative. In culture positive, 182 G-ve bacteria and 27 G+ve bacteria, 23 fungus were isolated. In 182 G-ve bacteria, Klebsiella sps (24%) as the predominant pathogens followed by Pseudomonas sps(20%), Enterobacter (10%), Acinetobacter sps (3%), E.coli (6%). Of the identified pathgoens, 50% were found to be ESBL producers and 31 % were MDR, 10% Acinetobacter and 9% E.coli. Colistin was highly susceptible (60-80%), followed by Imipenem and Meropenen (50-60%), Cefaperazone/Sulbactum (10-15%) and Piperacillin/Tazobactum (5-40%) against Gram negative bacteria. From the above results it can be conclude that Klebsiella sps and Pseudomonas sp was the most common pathoges associated with COPD patient of intensive care unit hospitalized elder in private hospital, Colistin and Imipenem is an drug of choice to treat the infection caused by carbapnemase producing multi-drug resistant gram negative bacteria.

Keywords: COPD, Gram negative bacteria, ESBL.