Abstract

ISOLATION AND IDENTIFICATION OF MICROORGANISMS FROM BOVINE MASTITIS INFECTED MILK SAMPLES AND THEIR ANTIBIOGRAM

Mahenthiran R.* and Michael A.

Abstract

Bovine Mastitis is an inflammatory reaction of the udder tissue which is caused by the bacterial infection. Bovine mastitis is one of the devastating disease causing heavy economic loss to the dairy industry in worldwide. The present study investigated the current status of clinical mastitis among dairy cattles in Coimbatore. The mastitis infected milk samples (n=64) were assessed at based on positive reaction to California mastitis test (CMT)(Strong positive 35.93%), surf field mastitis test (SFMT)(Strong positive 40.62%), NaOH test (Strong positive 37.5%),PH and bacteriological evaluation of milk samples. The major pathogens isolated from the milk samples were S.aureus, E.coli, Klebsiellasp, coagulase negative staphylococci (CNS), Streptococcus species, Bacillus sp, Pseudomonas species and Proteus sp. Antibiogram studies were also conducted for the isolates by using twelve antibiotics like amoxycillin, methicillin, amikacin, kanamycin, endrofloxacin, penicillin-G, Vancomycin, chloramphenicol, streptomycin, gentamicin, tetracycline and oxytetracycline. which were used in the regular veterinary practice for the treatment of mastitis. The bacterial examination revealed that the predominant cause is Staphylococcus aureus (26.50%). The most effective antibiotic was chloramphenicol (63.68%). Antibiogram studies of mastitis pathogens are important to suggest suitable antibiotic treatment to provide quality milk to the consumers and to prevent antibiotic resistance, potential health risk for humans.

Keywords: Bovine mastitis, CMT, SFMT, NaOH, Bacterial pathogens, Antibiogram.