STUDY OF TOXICITY PARAMETERS ON COMBINED HERBAL EXTRACT OF ASYSTASIA GANGETICA, FICUS RECEMOSA AND MORUS INDICA USING EXPERIMENTAL RATS.
Somanathan S.S.*, Ranganayakulu D. and Jayaveera K.N.
Abstract
Aim of this study is to create a data on the safety and toxic levels of
this combined extract preparation for further screenings and usages.
Mostly single plant extract toxicity and safety study is being done, but
required data for this combined extract preparation-OECD 425
guideline is followed to conduct toxicity effect for this study. This
guideline was preferred to conduct the toxicity, due to 1) minimize the
number of animals to estimate LD50 and confidential interval (CI).
estimation. 2) also the guideline OECD 425 was concurrently under
taken with revision of test guidelines 420 and 423 for oral toxicity
testing. The ethanolic extract of Asystasia gangetica (A- Acanthacaea),
Ficus recemosa (F-Moraceae) and Morus indica (M-Moraceae) evaluated for acute toxicity
(AFM) in adult wistar rats. The ethanolic extract of AFM (A+F+M) was administered as
175(50+60+65)mg, 500(150+175+175)mg, 1500(450+525+525)mg and
5000(1500+1700+1750)mg. The first group animal is dosed a step below (~ly,-3.2 factor) the
best preliminary estimation of LD 50 (
Keywords: Safety, toxicity, combined extract, OECD 425, Asystasia gangetica, Ficus recemosa, Morus indica.
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