Abstract

EVALUATION OF ANTI-UROLITHIATIC EFFECT OF AQUEOUS EXTRACT OF PARSLEY (PETROSELINUM SATIVUM) USING ETHYLENE GLYCOL-INDUCED RENAL CALCULI

Gehan Salah Eldin Moram*

Abstract

Objective: The present study was undertaken to evaluate the antiurolithiatic effect of petroselinum sativum (Parsley ) at a dose ( 25 mg /kg body weight /day) on ethylene glycol induced renal calculi in male albino rats and was compared with cystone as reference drug at a dose (750mg/kg body weight /day). Urolithiasis was induced by ethylene glycol (0.75% v/v) and ammonium chloride (1% w/v) in drinking water. Methods: Animals were divided into five groups; namely, healthy control rats, Lithiatic control rats, Cystone treated, preventive regimen and curative regimen. Results: Treatment with the parsley aqueous extract (preventive or curative regimen )restored all the elevated biochemical parameters including serum and urine (calcium, oxalate, phosphate , protein , creatinine and BUN ).In addition, parsley aqueous extract groups restored the urine pH to normal and significantly increased the urine volume and magnesium (P < 0.05) associated with improved in renal function. Lipid peroxidation in the kidneys was increased following the lithogenic treatment; however, preventive and curative regimens with parsley reduced the elevated levels of MDA, this evident attributed with the increased kidney reduced glutathione and antioxidant enzymes. Microscopic and histopathological examinations confirmed the above results and revealed that the potential prophylactic and curative parsley aqueous extract on inhibition renal stone associated with decreased calcium kidney content. In conclusion: Results of this study suggest the presence of antiurolithic effects in parsley against calcium oxalate stones mediated through a combination of antioxidant, diuretic, urinary alkalinizing and hypocalciuric effects.

Keywords: stones, antiurolithiatic, Parsley, antioxidant, diuretic, Cystone.