Abstract

IN VITRO ANTIINFLAMMATORY ACTIVITY OF METHANOL EXTRACT OF HOPEA ODORATA (ROXB.) LEAVES.

Muhammad Abdulla Al Noman, Fahima Zaheed1, Abul Hasanat, Md. Saiful Islam Arman, Sumya Afroze, Md. Shohel Hossain, Fahmida Abdullah Tuly, Mosarrof Hossain, Md. Najem Uddin, Mohammad Shah Hafez Kabir*

Abstract

Objective: To examine the anti-inflammatory effect of methanol extract of Hopea odorata (Roxb.) leaves. Methods: Leaves of Hopea odorata was extracted with pure methanol (MEHO), which is tested for in vitro anti-inflammatory activity found by protein denaturation. Result: The production of auto antigen in certain arthritic disease may be due to denaturation of protein, membrane lysis and proteinase action. The maximum percentage inhibition of protein denaturation membrane stabilization and proteinase inhibitory action were observed as H. odorata (80.13%, 75.33%, 67.43%, 52.83%, 38.31%, 22.29%) at 400, 200, 100, 50, 25, 12.5 μg/ml respectively. Conclusion: From our study, it can be concluded that in vitro anti-inflammatory activity of the leaves extract of H. odorata might be attributed to the presence of different phytochemicals. These experimental findings support the traditional use of this plant for the treatment of various ailments especially against pain and inflammatory conditions.

Keywords: Hopea odorata, methanol extract, anti-inflammatory, protein denaturation.