Abstract

EFFECT OF DERMAL EXPOSURE OF PREGNANT RATS TO DIAZINON ON THE TESTIS OF MALE OFFSPRING

Mufedah Ali Jawad, Ban Thabit Al-Ani, Muayad Sraibet Abbod, Mohammad Oda Selman*

Abstract

Diazinon is an insecticide that belongs to a group of chemicals known as organophosphates. It is used in agriculture to control insects on fruit, vegetable, nut and field crops, as well as it is used to make ear tags for cattle. The pure chemical (100% Diazinon) is colorless and practically odorless oil. Diazinon may enter the environment during the manufacturing process, but most environmental contamination comes from agricultural and household application of the chemical to control insects. Diazinon is often sprayed on crops and plants, so small particles of the chemical may be carried away from the field or yard before falling to the ground. Aim of the study: To study the histological changes that may occur in offspring rat testis resulting from dermal exposure of the pregnant rats to diazinon solution throughout pregnancy. Material and method: The study was performed on 30 pregnant albino rats (Rattus rattus norvegicus albinus). They were divided into two groups (15 in each). The first group was daily immersed in distilled water for 4 minutes throughout their pregnancy until giving birth and their male offspring were considered as control group. The second group was immersed in diazinon solution for the same period as done in the first group and their offspring was considered as experimental group. After birth the male off spring were sacrificed and the testes were taken for histopathological examination. Diameter of seminiferous Tubules and interstitial space were measured in both groups and comparison between the two results was done. Results: Significant (P

Keywords: Diazinon, insecticides, organophosphorous, testes.