Abstract

ANTIMICROBIAL ACTIVITY AGAINST GANGRENE USING SILVER NANOPARTICLES SYNTHESIZED BY COLEUS AROMATICUS

J. Shanmuga Priya¹, K. Poornima*², C. Priyadarshini³, S. Yamini Sudha Lakshmi, J. Madhusudhanan

Abstract

Biologically synthesized nanoparticles have been widely used in the field of nanoscience and medicine. In the present investigation, nanoparticles are prepared using medicinally important plant like Coleus aromaticus. 1mM and 3mM silver nitrate (AgNO3) solution and the corresponding leaf extracts in ratio 1:9 were used to synthesize the silver nanoparticle. The synthesized silver nanoparticles were characterized using UV-VIS spectroscopy, Scanning Electron Microscope (SEM), Transmission Electron Microscope (TEM) and Fourier Transform Infra-red Spectroscopy (FTIR). The appearance of dark brown colour and the UV absorbance for Coleus aromaticus ranged at 460 nm confirms the synthesis of silver nanoparticles. SEM was used to determine the size of the nanoparticle and it was found to be in the range of 20- 60 nm. The aim of the study is to determine the antibacterial and antifungal activity of the synthesized silver nanoparticles from the leaf extracts of Coleus aromaticus. The most predominantly found microbial species in gangrene are Pseudomonas auringenosa, Escherichia coli, Klebsiella pneumoniae, Clostridium perferingens, Enterococcus faecalis, Candida albicans, Staphyllo coccus. Agar well diffusion method was used to check the activity of silver nanoparticle against gangrene causing microbes. The important outcome of the study will be the development of value added products from above mentioned medicinal plants of India for biological and nanotechnology based industries.

Keywords: Coleus aromaticus, Clostridium perferingens, Candida albican, silver nanoparticle, SEM, TEM, FTIR.