Abstract

SODIUM SELENITE ATTENUATES NICOTINE-INDUCED OXIDATIVE STRESS IN RAT

Sankar Kumar Dey*, Durga Pada Dolai, Debasis Mandal, Balaram Das, Sandeep Kumar Dash and Somenath Roy

Abstract

Nicotine, a major toxic component of cigarette smoke has been identified as a major risk factor for different diseases. In the present study, we evaluated the protective effects of sodium selenite on lipid peroxidation and antioxidants status against nicotine treated liver and kidney toxicity. A group of male Wistar rats were induced by subcutaneous injection of vehicle, nicotine (2mg/kg body weight/day) and nicotine plus sodium selenite (0.5 mg/kg of diet) for the period of 15 days. Measurement of biochemical marker enzyme like lactate dehydrogenase (LDH), lipid peroxidation (MDA), conjugated dienes (CD) and antioxidants were used to monitor the antiperoxidative effect of sodium selenite. The decreased biochemical marker enzyme as well as increased lipid peroxides and conjugated dienes in liver and kidney of nicotine treated rats was accompanied by a significant decrease in the levels of glutathione (GSH and GSSG) and the activities of glutathione peroxidase (GPx), glutathione reductase (GR), glutathione-s-transferase (GST), superoxide dismutase (SOD) and catalase (CAT). Dietary co-administration of sodium selenite significantly supplements the biochemical marker enzyme, lipid peroxidation and enhanced the antioxidant status. The results of the present study suggest that sodium selenite exert its protective effect against nicotine-induced liver and kidney toxicity by modulating the biochemical marker enzyme, lipid peroxidation and augmenting antioxidant defense system.

Keywords: Nicotine, Sodium selenite, Tissues, Oxidative stress.