Abstract

THROMBOLYTIC EFFECT OF SOME ANTIBIOTIC DRUGS: IN VITRO AND IN SILICO APPROACH.

Mohammad Shah Hafez Kabir, Mohammed Shamim Hasan, Md. Giash Uddin, Gour Saha, Md. Abdullah-Al-Mamun and Nishan Chakrabarty*

Abstract

The present study aims to investigate the thrombolytic effect of some antibiotic drugs by in vitro clot lysis method and in silico molecular docking used to identify whether these drugs interact with the responsible protein (tissue-type plasminogen activator). In vitro clot lysis model was used to observe the thrombolytic effect of Amoxicillin, Azithromycin, Aztreonam, Cephalospirin, Ciprofloxacin, Phenoxymethylpenicillin and Rifampin drugs provided the clot lysis 32.03±1.78%, 38.25±3.41%, 33.49±2.03%, 36.96±2.47%, 33.94±4.2%, 34.32±1.77% and 40.41±2.15% clot lysis, respectively. Reference drug streptokinase exhibited 78.70±0.92% clot lysis. A wide range of docking score found during molecular docking by CPI server. Amoxicillin, Azithromycin, Aztreonam, Cephalospirin, Ciprofloxacin, Phenoxymethylpenicillin and Rifampin drugs showed the docking score -8.0, -7.3, -7.2, -7.2, -6.8, -7.4 and -9.1, respectively. Rifampin possessed highest clot lysis effect and also showed best docking score among the antibiotics. Further in vivo investigation need to identify the thrombolytic effect of these antibiotics and also require making out the mechanism of them as thrombolytic agents.

Keywords: Antibiotic drugs, Molecular docking, Amoxicillin, Ciprofloxacin, Rifampin.