Abstract

CHARACTERISATION, INVITRO AND INSILICO ANTIDIABETIC ACTIVITY IN THE LEAF EXTRACTS OF MANGIFERA INDICA

Khoushika Raajshree R.* and Chitra P

Abstract

Diabetes mellitus is a metabolic disorder characterized by a loss of glucose homeostasis with disturbances of carbohydrate, fat and protein metabolism. Several phytocompounds are known to modulate glucose metabolism by inhibition of carbohydrate digesting enzyme like α- amylase. Leaves of Mangifera indica were subjected to qualitative tests and quantitative total phenolic content assay. It was found that the ethanolic extract had high phenolic content. Ethanolic extract also had maximum DPPH free radical scavenging activity and invitro α- Amylase Inhibition activity. GC-MS analysis of the ethanolic extracts of the samples gave several compounds which were docked against the target Human Pancreatic Alpha-Amylase. Ethyl octadecanoate was found to be an active compound through insilico docking with the least E value of – 6.35 kcal/mol. This result may pave the way to modulate glucose metabolism using phytocompounds and thereby may lead to the development of more effective chemical entities with antidiabetic property.

Keywords: Glucose homeostasis, ?-amylase, Human Pancreatic Alpha-Amylase, Ethyl octadecanoate, docking.