Abstract

IN-VITRO ANALYSIS OF CASPASE-3 IN VANILLIC ACID TREATED NON- SMALL LUNG CANCER CELL LINE NCI-H460

Pabbisetty.P.B.S.Kumar, Dr. K. Ammani

Abstract

A dietary Phenolic compound Vanillic acid (VA) found in some plants, fruits and also an intermediate in the production of vanillin from ferulic acid. Researchers completed the experiments of its anti-oxidant, anti-microbial and anti-malarial activities. However, its effect on lung cancer cell lines has not yet been fully studied: Caspase 3 was measured at the mRNA level using reverse transcription-PCR and at the protein level using both Western blotting and ELISA. In this experiment, tests used to analyze were basic, simple and rapid but effective, which were Reverse Transcription Polymerase Chain Reaction, ELISA and western blotting. VA treatment on NCI-H460 cells showed that detectable and increasing levels of Apoptotic marker Caspase-3. We observed a concentration-dependent alteration of apoptotic marker caspase-3 in treated NCI-H460 cells, hence, we may conclude that this compound may be useful as therapeutic agent. However, further in vitro and in vivo experiments must be carried out to know its complete effect.

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