Abstract

HPTLC METHOD FOR ESTIMATION OF FLAVONOIDS AND PHENOLIC ACID IN CLITORIA TERNATEA ROOT EXTRACT

Saravanan J., Swathi K. P. and R. Arivukkarasu

Abstract

Clitoria ternatea is a well known bioactive plant used in traditional system of medicine. The aim of the present study is to evaluate the ethanolic extract of white flowered variety of Clitoria ternatea roots (EECT) with standard flavonoid markers such as rutin, quercetin, gallic acid by HPTLC techniques. The chromatographic separation was carried out on precoated silica gel 60F254 aluminium plates using mixture of Toluene- Ethyl acetate-Formic acid- Methanol (3 : 6 : 1.6: 0.4v/v) as mobile phase and densitometric evaluation of spots was carried out at 254 nm using Camag TLC Scanner-3 with win CAT 1.3.4 version software. The identity of the constituents in the ethanol extract of Clitoria ternatea were confirmed by overlaying their UV absorption spectra with the standard markers rutin, quercetin and gallic acid at 254 nm. The percentage of active constituents present in the extract was calculated from the peak area. From the result of HPTLC analysis it was evident that EECT contains phytoconstituents like gallic acid (0.8%) and rutin (0.06%).

Keywords: HPTLC, Clitoria ternatea, rutin, gallic acid.