Abstract

ASSESSMENT OF SIZE BASED ORAL TOXICITY OF SILVER NANOPARTICLES ON SERUM LIPID PROFILE, LIVER AND KIDNEY FUNCTION OF MALE WISTAR RATS

Utkarsh Kaushik and S. C. Joshi*

Abstract

Nanotechnology has received unprecedented growth and height which has revolutionized the consumer products, medical and industrial applications, thereby making it more compact, feasible and appropriate. But uncurbed use of nanoparticles and nanomaterials has led to its release into the environment and its exposure to human beings. Present study aimed to quest the repercussion of PVP coated silver nanoparticles (20 nm and 40 nm) administered orally at a dose level 1 and 2 mg/kgb.wt./day for 45 days on male albino rat. On 46th day body weight was measured and autopsies were performed to collect serum for anaylsis. Organ like liver and kidney were used for histopathologic examination. Group recevied high dose showed highly significant decline. Liver weight was found to be affected. The mild to severe alteration in serum profile was obtained indicating liver damage whereas kidney damage was clear from blood urea and creatinine level in serum. These results were supported by histopathology observations of liver and kidney. Thus it can be concluded from data that the oral administration of AgNPs (20 nm and 40 nm) drive to hepatic and renal toxicity in experimental groups at such a low dose under in vivo condition. Catastrophic effects shown by AgNPs (20 nm) are more compared to AgNPs (40 nm).

Keywords: AgNPs, serum lipid profile, liver, kidney, histopathology.