Abstract

KINETIC SPECTROPHOTOMETRIC DETERMINATION OF DRUGS BASED ON OXIDATION BY ALKALINE KMnO4

G. Sumalatha and G. Venkateshwarlu*

Abstract

Simple, accurate and precise spectrophotometic methods for quantitative determination of five drugs viz., piperacillin (PIP), Argatroban (ARG), Levocetrizine (LCTZ), Methocarbamol (MET), Diclofenac Sodium(DCS), have been developed based on oxidation of the drugs by alk. KMnO4. Kinetics of the oxidation reaction is followed spectrophotometrically, as one of the reaction product, Mn(VI), absorbed at 610 nm. Initial rate and fixed time method are used for the construction of calibration curves Beer’s law is obeyed in the range 20-120 μg ml-1 for PIP; 5-30 μg ml-1 for ARG ;10-60 μg ml-1 for LCTZ; and 10-60 μg ml-1 for MET; 7-42 μg ml-1 for DCS. Recovery studies using pure samples and formulations in the Beer’s Law limits have been carried out. Excellent recoveries indicate the methods are accurate and precise. The methods have been validated in terms of ICH guidelines. Statistical analysis in terms of student’s t- test and variance F- tests demonstrate high accuracy and precision and suggest the methods can be applied in bulk drug and pharmaceutical industries.

Keywords: Spectrophotometry, Quantitative determination, Alkaline KMnO4, Drugs.