Abstract

PHYTOCHEMICAL AND BIOLOGICAL STUDY OF DIOSPYROS KAKI L. GROWING IN EGYPT

Hala abozaid, Abeer Moawad*, Elham Amin, Mona Hetta, Marawan Shabana

Abstract

The leaves of Diospyros kaki L. is a traditional medicine used in Japan for the treatment of hypertension, angina and internal hemorrhage. The present study aims to investigate the antitumor and antimicrobial activities of the ethanolic extract (EE), unsaponifiable matter (USM), ethyl acetate extrac (EtOAc) of this plant. Moreover, seven compounds were isolated from the leaves and their activities were evaluated. The anticancer activities were studied using MCF-7 breast cancer cell line and HeLa-cervical carcinoma cell line. The antimicrobial activity was studied by in vitro diffusion agar method. The EE, USM and EtOAc extract showed anticancer activities against MCF-7 breast cancer cell line (IC50=50μg/mL, 25.4μg/mL and 19.8 μg/mL respectively) and HeLa-cervical carcinoma cell line (IC50=46.2μg/mL, 7.8 μg/mL and 16.7μg/mL respectively). Among the isolated compounds, lupeol (1) was the most active showing anticancer activity against MCF-7 breast carcinoma cell line and HeLacervical carcinoma cell line (IC50 = 20.7μg/mL and 23.7μg/mL respectively). The EtOAc extract showed strong antimicrobial activity against all tested microorganisms with zones of inhibition ranging from 13.6 to 24.6 mm at a concentration of 5mg/mL. Isolated compounds showed different activities against the tested microorganisms, with kaempferol (5) being the most active.

Keywords: Diospyros kaki L.,E benaceae, anticancer, antimicrobial