Abstract

BIOAUTOGRAPHY GUIDED ANTIFUNGAL INVESTIGATION OF ADHATODA VASICA (NEES)

Elham Amin*, Nurhayat Tabanca and David E. Wedge

Abstract

In a research program aimed at identifying natural fungicides, seven plant extracts from Egyptian flora were evaluated for their antifungal activity using direct bioautography assay. Based on the bioautographic results, Adhatoda vasica was chosen for further investigation. Bioassay-guided isolation study was performed on hexane extract and resulted in the isolation of one compound, lupeol. This compound was evaluated for activity against plant pathogens Colletotrichum fragariae, C. gloeosporioides, C. acutatum, Botrytis cinerea, Fusarium oxysporum, Phomopsis obscurans and P. viticola in dose-response bioassays at 25, 50 and 75 μM. Lupeol showed 55% growth inhibition in a 96-well microdilution broth assay against Botrytis cinerea.

Keywords: Adhatoda vasica, plant pathogens, bioautography, 96-well micro-dilution broth assay