Abstract

A REVIEW ON DIFFERENT SCREENING METHODS TO IDENTIFY AND ISOLATE POTENT LIPASE PRODUCERS

Arun Kumar Sharma, Vinay Sharma* and Jyoti Saxena

Abstract

Lipases belonging to the class Hydrolases catalyze hydrolysis of insoluble triacylglycerols to generate free fatty acids, mono and diacylglycerols and glycerol. Lipases found promising application in various industries such as detergent, agrochemical, paper, chemical processing, dairy, pharmaceuticals, oleochemical, cosmetics, polymer synthesis, synthesis of surfactants and personal care products. Presently, the demand of the industries for novel sources of lipases with diverse catalytic features promotes the isolation and screening of new lipolytic microbial strains. Present review is focused on various methods of screening available for identification and isolation of lipolytic microorganisms from natural sources such as soil.

Keywords: Lipases, triacylglycerol, screening, lipolytic strains, isolation.