Abstract

GC- MS ANALYSIS OF LEAVES AND FLOWERS OF POGOSTEMON QUADRIFOLIUS (BENTH.) F.MUELL. (LAMIACEAE)

Jisha M.*, Zeinul Hukuman N.H. and Leena P.

Abstract

The aim of the present study is to determine the phytoconstituents present in the petroleum ether, acetone and methanol extracts of leaves and flowers of Pogostemon quadrifolius using GC –MS. The shade dried powdered leaves and flowers of Pogostemon quadrifolius were extracted sequentially using petroleum ether, acetone and methanol in increasing order of polarity using soxhlet apparatus. GC- MS analysis were performed using Thermo Scientific Trace 1300 Gas chromatograph equipped with ISQ- QD Mass spectrometer. GC MS analysis revealed 37 phytochemical compounds in the petroleum ether extract, 30 compounds in the acetone extract and 6 compounds in the methanol extract of leaves. 2,4,6 Trimethoxy acetophenone was the major phytoconstituent in petroleum ether (25.95%) and acetone extracts (77.96%) of leaves where 1-Methoxy-4,4a,5,6,7,8-hexahydro-2(3H)-naphthalenone (66.65%) was the major constituent in methanol extract of leaves. 44 phytochemical compounds were found present in the petroleum ether extract, 12 compounds in the acetone extract and 24 compounds in the methanol extract of flowers of Pogostemon quadrifolius. 3 methyl pentane (57.39%) was the major phytoconstituent in petroleum ether extract of flowers, where n – butane (53.26%) and geranyl vinyl ether (44.64%) were the major compounds in acetone and methanol extracts.

Keywords: Pogostemon quadrifolius, phytoconstituents, GC MS analysis, leaves, flowers.