Abstract

THE PROTECTIVE EFFECT OF PHYLLANTHUS EMBLICA AGAINST CYCLOPHOSPHAMIDE INDUCED ABERRANT SPERMS IN MICE

Keshava Rao, K. Rudrama Devi K.* and Minny Jael P.

Abstract

A number of antineoplatic drugs are used to combat with different types of cancer, and had been shown to be cytotoxic. Cyclophosphamide is one of most commonly used in malignant lymphomas. The antioxidants such as phyllanthus emblica inhibits the toxicity of mutagens/carcinogens in animals and Humans. The protective effects of phyllanthus emblica in cyclophosphamide induced toxicity in swiss male mice was evaluated using sperm morphology assay. Two experiments were conducted in germ cells of Swiss male mice. The cauda epidymis was collected from control and exposed group of animals after five weeks of treatment schedule and screened for presence of various types of sperm head abnormalities such as amorphous, banana hammer head etc., The animals were treated with 170, 380 and 640 mg/kg of phyllanthus emblica showed as non mutagenic where as the cyclophosphamide induced significant increase in the percentage sperm head abnormalities but when primed with AA there is a significant decrease in the percentages of abnormal sperms. Phyllanthus emblica protects the cyclophosphamide induced genotoxicity in germ cells of mice. Hence phyllanthus emblica supplementation is safer in chemotherapeutic strategy.

Keywords: phyllanthus emblica, genotoxicity, cyclophosphamide, sperm morphology.