Abstract

OXIDANT, ANTIOXIDANTS AND SOME BIOCHEMICAL PARAMETERS IN PATIENTS WITH TYPE 2 DIABETIC NEPHROPATHY

Ala'a Waleed Abbas*, Waleed Hameed Yousif, Khaleed Jaleel Khaleel

Abstract

This work was conducted to study the role of oxidative stress and some biochemical parameters in type 2 diabetic nephropathy patients. Fifteen type 2 diabetic nephropathy and ten type 2 diabetic patients (at the age 57-68 years) attending to the abd- Almajid private hospital were recruited for this study. For the purpose of comparison, seven healthy controls subjects matched for age were also included. Random blood sugar, serum urea, serum creatinine, glycosylated hemoglobin, serum complements 3 and 4, serum malondialdehyde, serum glutathione and serum catalase activity were measured in blood. Microalbumin, creatinine and albumin / creatinine ratio were measured in urine. The results showed increase in random blood sugar in diabetic and diabetic nephropathy patients at the ages of 57-68 years. No change was observed in serum urea and creatinine concentrations in diabetic patients, whereas an increase was demonstrated in diabetic nephropathy patients. The percentages of glycosylated hemoglobin increased significantly in diabetic and diabetic nephropathy patients. No changes were seen in serum complements 3 and 4, malondialdehyde and glutathione concentrations in diabetic and diabetic nephropathy patients. A significant increase in serum catalase activity was demonstrated in diabetic and diabetic nephropathy patients. No change was observed in urine microalbumin concentration in diabetic patients, while a significant increase was seen in diabetic nephropathy patients. Urine creatinine concentrations in diabetic and diabetic nephropathy patients showed no change. Albumin / creatinine ratios showed increase in diabetic nephropathy patients. No change was observed in albumin / creatinine ratio in diabetic patients. It is concluded thatdiabetic patients demonstrated hyperglycemia, an increase in the percentages of glycosylated hemoglobin and catalase activity with an associated no changes in serum complements 3, 4, MDA and GSH. Diabetic nephropathy patients showed more profound biochemical changes than diabetic patients as reflected by hyperglycemia, an increase in serum urea, creatinine, the percentages of glycosylated hemoglobin, catalase activity, urine creatinine and A/C ratio with an associated microalbuminuria at the different age groups. Serum complements 3 and 4, MDA and GSH remained unchanged.

Keywords: Random blood sugar, serum urea, serum creatinine, MDA, GSH, Catalase, HbA1c.