Abstract

ISOLATION AND BIOLOGICAL EVALUATION OF (24R) STIGMAST- 5-EN-3-OL FROM CASSIA FISTULA BARK.

Brinda Bolar, Jennifer George and Gangadhar A. Meshram*

Abstract

Cassia fistula is known as the Golden rain tree which belongs to Fabaceae family. In Ayurvedic medicine it is well known as a disease killer. The extracts of cassia fistula are widely used as traditional antidiabetic medicines. One of the therapeutic approach for preventing diabetes mellitus-2 is to retard the glucose absorption in the blood via inhibition of glucosidase viz glucoamylase and α-Amylase. In the present study (24R) Stigmast-5-en-3-ol was isolated from the bark of cassia fistula. The structure was elucidated on the basis of MS, NMR and IR. (24R)Stigmast-5-en-3-ol exhibited potent inhibitory activity against glucoamylase and α-amylase with IC50value 5.8 μg/mL and 7.198 μg/mL respectively. The antioxidant activity was evaluated by DPPH method. The results indicated that (24R) Stigmast-5-en-3-ol possess considerable antioxidant activity. The highest radical activity was detected at 71.42 μg/mL. As a result (24R) Stigmast-5-en-3-ol isolated from cassia fistula can be used for antioxidant therapy during management of Diabetes Mellitus Type-2 as well.

Keywords: Cassia fistula, (24R) Stigmast-5-en-3-ol, glucoamylase, ?-amylase, antioxidant activity.