Abstract

ANTIFUNGAL ACTIVITIES OF CALOTROPIS GIGANTEA AGAINST DOMINANT FUNGAL PATHOGENS OF MONUMENT WITH REFERENCE TO BHAND DEOL TEMPLE OF CHHATTISGARH

*Sanjay Prasad Gupta

Abstract

Four common and dominant species of fungi viz A. niger, Rizopous, Cladpsporium and Curvularia lunata isolated from archaeological site were subjected to laboratory experiment involving in vitro control of the fungal species using medicinal plant extracts. Aqueous leaves extract at 10%, 20%, 30%, 40% and 50% with the control (basal medium) concentrations tested on potato dextrose agar (PDA) for activity against mycelium growth were determined at 26±10C with three replicated plates. Fungal growth values recorded were generally low compared with the control (without extract petri plate). Inhibitory action of the extract on fungal growth increased with increases in concentration of extract. A study was carried out to evaluate the antifungal properties of aqueous extract of Calotropis gigantea (Aak plant) on common and dominant fungal species, isolated from Bhand Deol temple at Arang of Chhattisgarh state using the well in PDA media. The in vitro studies have been performed by using aqueous leaf extract of Calotropis gigantea (Aak plant). Extract showed antifungal activity. Different concentration of extract solutions prepared and standardized for the study. It was found that the 3 ml of 37% and 7 ml of 47% extracts were effective in reducing the mycelial growth of A. niger and Curvularia lunata respectively whereas not effective of Aak extract for Cladosporium and Rizopous. Concentrations of extract which inhibit 75% and above having known as effective in this study. Plant extracts readily available and affordable and environmentally friendly in the control of fungal disease.

Keywords: Mycelial growth; fungal species; Calotropis gigantea (Aak plant). plant extract; antifungal activity; concentration and culture media.