Abstract

PRODUCTION AND OPTIMIZATION OF L-GLUTAMINASE FROM VIBRIO SP. M9 ISOLATED FROM MAHABALIPURAM MARINE SEDIMENTS

Abstract

In the present study L-glutaminase producing bacteria were isolated from sediment samples collected from Mahabalipuram, Parangipettai and Sundarban mangrove ecosystems. L- glutaminase from selected 15 bacterial isolates was produced by submerged fermentation. Bacterial strain M9 isolated from Mahabalipuram marine sediments showed maximum L- glutaminase activity in semiquantitative assay. The crude enzyme from potential strain M9 was produced and partially purified by ammonium sulphate precipitation and dialysis. In quantitative assay, the partially purified preparation showed 28.7U/ml of enzyme activity.In SDS PAGE the enzyme showed two bands with 45 and 250 kDa respectively. Of the various Physic-chemical parameters tested,pH 7, temperature 35°C and 3.5% NaCl concentration was found to influence Lglutaminase production by the strain M9.The potential marine bacterial isolate was identified as Vibrio sp., based on its morphological, cultural, biochemical and physiological characteristics.

Keywords: Marine bacteria, L-glutaminase, Vibrio sp