Abstract

CHLOROPHYLL AND MANUKA HONEY - SUITABLE ALTERNATE FOR VISA CAUSING POST CESAREAN WOUND INFECTION

*Pallavi Jayavanth, Getaneh A., Mohammedaman M., Ashenafi A. Mathan M., Yasmeen M.

Abstract

Antibiotic resistance and post cesarean wound infections have been a continuous problem for clinicians. Alternate low cost natural antibacterial agents are evaluated in parallel for their efficacy due to escalating antibiotic resistance. In view of this, a study was conducted to record the efficacy of chlorophyll and manuka honey against vancomycin intermediate Staphylococcus aureus (VISA). Confirmed VISA isolates from post cesarean wound infection patients were collected from infection control practitioner and subjected to antibacterial assay against different concentrations of chlorophyll and manuka honey as per standard procedure. Stock solutions of chlorophyll and manuka honey were prepared at 1% w/v concentration with dilution range from 25μl/ml to 250μl/ml and subjected to minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) against VISA. MIC and MBC recorded for chlorophyll and manuka honey was 150μl/ml, 100μl/ml and and MBC 200μl/ml, 150μl/ml respectively. Chlorophyll and manuka honey demonstrated promising results to use as alternate antibacterial agent against VISA causing post cesarean wound. Pharmacological preparation of chlorophyll and manuka honey embedded in bandages could aid as effective therapeutic alternate to treat post cesarean wound infection.

Keywords: Chlorophyll, manuka honey, drug resistance, vancomycin, VISA, post cesarean wound.