Abstract

EVALUATION OF PHYTOCHEMICAL PROFILE AND ANTIOXIDANT ACTIVITY OF EQUISETUM HYEMALE L

Garima Pandey* and Sayyada Khatoon

Abstract

Objective: Equisetum hyemale (EH) has been traditionally used for bone healing.This study focussed on chemical profile and antioxidant potential of aerial part of EHwith a view to support its role in improving bone health. Methods: Thephytochemicals were identified and quantified using HPTLC. The analysis of macroand micro elements was done using inductively coupled plasma mass spectrometer.The antioxidant activity was studied using DPPH free radical scavenging, β-Carotene-linoleic acid assay and reducing power assay. Results: The HPTLC studiesof aqueous-ethanol (50%) extract of EH (AEEH) showed the presence of rutin (429.4μg. g-1), β-sitosterol (372.9 μg. g-1), lupeol (259.4 μg. g-1), diosgenin (163.1 μg. g-1),and ellagic acid (27.5 μg. g-1). The key mineral elements identified in the driedpowder of EH were iron, zinc, manganese, copper and chromium. The total phenoland total flavonoid content in AEEH were 332.32 mg.g-1 and 38.46 mg.g-1 dryextracts, respectively. The AEEH showed significant (p<0.05) scavenging of DPPHfree radicals, IC 50 was 0.081 mg.ml-1. ). The anti-lipid peroxidation activity was48.06% and 60.82 % at a concentration of 1.20 mg/ml for AEEH and α-tocopherolrespectively. The reducing power was recorded as 0.268 ± 0.045 for AEEH and 0.362 ± 0.043 for the standardBHA, at a concentration of 1.25 mg/ml. Conclusion: Our study justified a prospective role of the E. hyemale inbone healing, owing to the presence of various phytocompounds and significant antioxidant potential.

Keywords: Equisetum hyemale, Rutin, Diosgenin, Antioxidant, Bone healing.