Abstract

MOLECULAR CHARACTERIZATION OF ENDOPHYTIC FUNGUS BOTRYOTINIA FUCKELIANA FROM SARACA INDICA

V. Pushpa Rani*, J. Delphine Jaya Mary, M. Parimalam, G. Meena Kumari, and K. Deepak Kumar

Abstract

The present study was focused on molecular characterization of endophytic fungus from ethnomedically important plant Ashoka tree (Saraca indica) in view to amplify their DNA region. Endophytic fungus which affects the Ashoka tree leaves (Saraca indica) has been identified from this study by various techniques, which was already used by other fungus identification and molecular characterization method. Genomic DNA was isolated through Qualitative and Quantitative analysis. 18S rRNA region was amplified through PCR standardization. Based on the differences in RNA genes the fungus has been identified and classified through Ribotyping method. The name of the endophytic fungus was identified as Botryotinia fuckeliana from the percentage of similar endophytic fungus appeared in histogram. This study will help to discover the causes and preventive mechanism of (Botryotinia fuckeliana) endophytic fungus in Saraca indica.

Keywords: Botryotinia fuckeliana, Saraca indica, DNA, Molecular Characterization, 18S rRNA.