Abstract

ISOLATION AND CHARACTERIZATION OF CONSTITUENTS FROM THE LEAVES OF KALANCHOE CRENATA AND THEIR EVALUATION FOR ANTIOXIDANT AND ANTIMICROBIAL POTENTIAL

Aarti Rathour, Anjoo Kamboj*, Mandeep Kaur, Ajay Kumar Saluja

Abstract

Kalanchoe crenata (Adrews) Haw. is an ornamental plant belonging to the family Crassulaceae is probably native to Africa and drier parts of south-East Asia. Commonly known as “never die” or “Dog’s liver”, this plant is widely used in traditional medicine in the treatment of inflammation, earache, headache, asthma, palpitation, abdominal pain, convulsion and general debility. The present research includes isolation and characterization of chemical constituents and in vitro evaluation of antioxidant potentials by three different assay methods of isolated constituents from leaves of Kalanchoe crenata. Three constituents (α-amyrin, Stearyl alcohol, and β-sitoterol-d-glycoside) were isolated and the structures of these compounds have been established by spectroscopic methods (UV, 1H-NMR, IR, MS). All these constituents were isolated from this plant for the first time. These chemical constituents showed significant antioxidant and antimicrobial activity in dose dependent manner by different assay methods. The result obtained in the present study indicates that leaves of Kalanchoe crenata could be a potential source of natural antioxidant and antimicrobial. This also justified the traditional use of herb in preventing disease induced by oxidative stress and microorganism.

Keywords: Kalanchoe crenata, DPPH scavenging activity, Gram positive, Gram negative.